Enrico Malito, PhD


Education

Ph.D. Genetic and Molecular Sciences/Protein Crystallography, 2005
B.S. Biological Science, 2001

Jan 2005 - Ph.D. Protein Crystallography, graduation

University of Pavia, Italy 
Thesis title: ”Phenylacetone monooxygenase and cytokinin dehydrogenase: structures and enzymatic mechanisms”

2001-2005 - Ph.D. student in Protein Crystallography

Laboratory of Structural Biology 
Dept. of Genetics and Microbiology, University of Pavia, Italy 
supervisor prof. Andrea Mattevi

Sept 2001 - Degree in Biological Sciences

University of Pavia, Italy 
Thesis title: ”Crystallographic studies of 4-hydroxyacetophenone monooxygenase from Pseudomonas fluorescens”

2000-2001 - Thesis work on Protein Crystallography

Laboratory of Structural Biology 
Dept. of Genetics and Microbiology, University of Pavia, Italy 
coordinator prof. Andrea Mattevi

1998-2001 - Bachelor student in Molecular Biology 

University of Pavia, Italy

1994-1998 - Bachelor student in Biological Sciences 

University of Calabria, Cosenza, Italy

Jul 1994 - High School Diploma Scientific High School (Liceo)

Liceo Galileo Galilei 
Paola, Italy

Awards

Jul 2007 - American Hearth Association (AHA) postdoctoral fellowship

Apr 2007 - American Health Assistance Foundation (AHAF), Alzheimer’s Disease Research Grant


Research experience


Jan 2011-Present - Research Investigator

Novartis Vaccines & Diagnostics (NV&D)
Siena, Italy

2009-2010 - Visiting scientist

Genomics Institute of the Novartis Research Foundation (GNF)
San Diego, USA

2006-2008 - Postdoctoral fellow

Prof. Wei-Jen Tang’s Lab
Ben May Dept. for Cancer Research, The University of Chicago
Chicago, USA
American Health Assistance Foundation (AHAF) Alzheimer’s Disease Research Grant
American Heart Association (AHA) fellow

2005-2006 - Postdoctoral scholar

Prof. Arnon Lavie’s Lab 
Dept. of Biochemistry and Molecular Genetics, University of Illinois at Chicago
Chicago, USA

2001-2005 - PhD student

Prof. Andrea Mattevi’s Lab 
Dept. of Genetics and Microbiology, University of Pavia
Pavia, Italy

2000-2001 - BA thesis work and research experience

Prof. Andrea Mattevi’s Lab 
Dept. of Genetics and Microbiology, University of Pavia
Pavia, Italy

Publications



2014

Structure of the meningococcal vaccine antigen NadA and epitope mapping of a bactericidal antibody.
Malito E, Biancucci M, Faleri A, Ferlenghi I, Scarselli M, Maruggi G, Lo Surdo P, Veggi D, Liguori A, Santini L, Bertoldi I, Petracca R, Marchi S, Romagnoli G, Cartocci E, Vercellino I, Savino S, Spraggon G, Norais N, Pizza M, Rappuoli R, Masignani V, Bottomley MJ.
Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17128-33. doi: 10.1073/pnas.1419686111. Epub 2014 Nov 17.

Apo, Zn2+-bound and Mn2+-bound structures reveal ligand-binding properties of SitA from the pathogen Staphylococcus pseudintermedius.
Abate F, Malito E, Cozzi R, Lo Surdo P, Maione D, Bottomley MJ.
Biosci Rep. 2014 Nov 24;34(6). pii: e00154. doi: 10.1042/BSR20140088.


History of Diphtheria Vaccine Development
Rappuoli R, Malito ECorynebacterium diphtheriae and Related Toxigenic Species. 2014, pp 225-238


2013

CLONING, EXPRESSION, PURIFICATION, CRYSTALLIZATION AND PRELIMINARY X-RAY DIFFRACTION ANALYSIS OF SPYCEP, A CANDIDATE ANTIGEN FOR A VACCINE AGAINST STREPTOCOCCUS PYOGENES.
Abate F, Malito E, Falugi F, Margarit Y Ros I, Bottomley MJ.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Oct;69(Pt 10):1103-6. doi: 10.1107/S1744309113024871. Epub 2013 Sep 28.

COMPARISON OF CRM197, DIPHTHERIA TOXOID AND TETANUS TOXOID AS PROTEIN CARRIERS FOR MENINGOCOCCAL GLYCOCONJUGATE VACCINES.
Tontini M, Berti F, Romano MR, Proietti D, Zambonelli C, Bottomley MJ, De Gregorio E, Del Giudice G, Rappuoli R, Costantino P; Study Group.
Vaccine. 2013 Oct 1;31(42):4827-33. doi: 10.1016/j.vaccine.2013.07.078. Epub 2013 Aug 17.

MINING THE BACTERIAL UNKNOWN PROTEOME: IDENTIFICATION AND CHARACTERIZATION OF A NOVEL FAMILY OF HIGHLY CONSERVED PROTECTIVE ANTIGENS IN STAPHYLOCOCCUS AUREUS.
Schluepen C*, Malito E*, Marongiu A, Schirle M, McWhinnie E, Lo Surdo P, Biancucci M, Falugi F, Nardi-Dei V, Marchi S, Fontana MR, Lombardi B, De Falco MG, Rinaudo CD, Spraggon G, Nissum M, Bagnoli F, Grandi G, Bottomley MJ, Liberatori S.
Biochem J. 2013 Nov 1;455(3):273-84. doi: 10.1042/BJ20130540.
(*equally contributed)

DEFINING A PROTECTIVE EPITOPE ON FACTOR H BINDING PROTEIN, A KEY MENINGOCOCCAL VIRULENCE FACTOR AND VACCINE ANTIGEN.
Malito E, Faleri A, Lo Surdo P, Veggi D, Maruggi G, Grassi E, Cartocci E, Bertoldi I, Genovese A, Santini L, Romagnoli G, Borgogni E, Brier S, Lo Passo C, Domina M, Castellino F, Felici F, van der Veen S, Johnson S, Lea SM, Tang CM,Pizza M, Savino S, Norais N, Rappuoli R, Bottomley MJ, Masignani V.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3304-9. Epub 2013 Feb 8.


2012


STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF THE STAPHYLOCOCCUS AUREUS VIRULENCE FACTOR AND VACCINE CANDIDATE FHUD2
Mariotti P, Malito E, Biancucci M, Lo Surdo P, Mishra RP, Nardi-Dei V, Savino S, Nissum M, Spraggon G, Grandi G, Bagnoli F, Bottomley MJ.
Biochem J. 2012 Oct 31. [Epub ahead of print]

THE STRUCTURE OF CLOSTRIDIUM DIFFICILE TCDA-GT DOMAIN BOUND TO MN(2+) AND UDP PROVIDES INSIGHT INTO GLUCOSYLTRANSFERASE ACTIVITY AND PRODUCT RELEASE.

D'Urzo N*, Malito E*, Biancucci M, Bottomley MJ, Maione D, Scarselli M, Martinelli M.
(*equally contributed)
FEBS J. 2012 Jul 2.

STRUCTURAL BASIS FOR LACK OF TOXICITY OF THE DIPHTHERIA TOXIN MUTANT CRM197 
Malito E, Bursulaya B, Chen C, Surdo PL, Picchianti M, Balducci E, Biancucci M, Brock A, Berti F, Bottomley MJ, Nissum M, Costantino P, Rappuoli R, Spraggon G.
Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5229-34. Epub 2012 Mar 19



2011


RATIONAL DESIGN OF A MENINGOCOCCAL ANTIGEN INDUCING BROAD PROTECTIVE IMMUNITY
Scarselli M, Aricò B, Brunelli B, Savino S, Di Marcello F, Palumbo E, Veggi D, Ciucchi L, Cartocci E, Bottomley MJ, Malito E, Lo Surdo P, Comanducci M, Giuliani MM, Cantini F, Dragonetti S, Colaprico A, Doro F, Giannetti P, Pallaoro M, Brogioni B, Tontini M, Hilleringmann M, Nardi-Dei V, Banci L, Pizza M, Rappuoli R.
Sci Transl Med. 2011 Jul 13;3(91)

STRUCTURE ANALYSIS AND SITE-DIRECTED MUTAGENESIS OF DEFINED KEY RESIDUES AND MOTIVES FOR PILUS-RELATED SORTASE C1 IN GROUP B STREPTOCOCCUS
Cozzi R, Malito E, Nuccitelli A, D'Onofrio M, Martinelli M, Ferlenghi I, Grandi G, Telford JL, Maione D, Rinaudo CD.
FASEB J. 2011 Jun;25(6):1874-86


2010


SUPRAMOLECULAR ORGANIZATION OF THE REPETITIVE BACKBONE UNIT OF THE STREPTOCOCCUS PNEUMONIAE PILUS
Spraggon G, Koesema E, Scarselli M, Malito E, Biagini M, Norais N, Emolo C, Barocchi MA, Giusti F, Hilleringmann M, Rappuoli R, Lesley S, Covacci A, Masignani V, Ferlenghi I.
PLoS One. 2010 Jun 15;5(6):e10919

DESIGNED INHIBITORS OF INSULIN-DEGRADING ENZYME REGULATE THE CATABOLISM AND ACTIVITY OF INSULIN 
Leissring MA, Malito E, Hedouin S, Reinstatler L, Sahara T, Abdul-Hay SO, Choudhry S, Maharvi GM, Fauq AH, Huzarska M, May PS, Choi S, Logan TP, Turk BE, Cantley LC, Manolopoulou M, Tang WJ, Stein RL, Cuny GD, Selkoe DJ
PLoS One. 2010 May 7;5(5):e10504


2009


MOLECULAR BASIS OF CATALYTIC CHAMBER-ASSISTED UNFOLDING AND CLEAV- AGE OF HUMAN INSULIN BY HUMAN INSULIN-DEGRADING ENZYME 
           Manolopoulou M, Guo Q, Malito E, Schilling AB, Tang WJ
J Biol Chem. (2009) vol. 284 (21) pp. 14177-88

CELLULAR FUNCTIONS AND X-RAY STRUCTURE OF ANTHROLYSIN O, A CHOLESTEROL- DEPENDENT CYTOLYSIN SECRETED BY BACILLUS ANTHRACIS 
Bourdeau RW, Malito E, Chenal A, Bishop BL, Musch MW, Villereal ML, Chang EB, Mosser EM, Rest RF, Tang WJ
J Biol Chem. (2009) vol. 284 (21) pp. 14645-56


2008


MOLECULAR BASES FOR THE RECOGNITION OF SHORT PEPTIDE SUBSTRATES AND CYSTEINE-DIRECTED MODIFICATIONS OF HUMAN INSULIN-DEGRADING ENZYME 
           Malito E, Ralat LA, Manolopoulou M, Tsay JL, Wadlington NL, Tang WJ
Biochemistry. (2008) vol. 47 (48) pp. 12822-34

AMYLOID β-DEGRADING CRYPTIDASES: INSULIN DEGRADING ENZYME, PRESEQUENCE PEPTIDASE, AND NEPRILYSIN 
           Malito E, Hulse RE, Tang WJ
Cell Mol Life Sci. (2008) vol. 65 (16) pp. 2574-85

REVEALING THE MOONLIGHTING ROLE OF NADP IN THE STRUCTURE OF A FLAVIN- CONTAINING MONOOXYGENASE 
          Alfieri A, Malito E, Orru R, Fraaije MW, Mattevi A
Proc Natl Acad Sci U S A. 2008 May;105(18):6572-6577


2007


INSIGHTS FROM ATOMIC-RESOLUTION X-RAY STRUCTURES OF CHEMICALLY-SYNTHESIZED HIV-1 PROTEASE IN COMPLEX WITH INHIBITORS 
Johnson EC*, Malito E*, Shen Y*, Pentelute B, Rich D, Florián J, Tang WJ, Kent SB
(*equally contributed
J Mol Biol. 2007 Oct 26;373(3):573-86

MODULAR TOTAL CHEMICAL SYNTHESIS OF AN HIV-1 PROTEASE 
          Johnson EC, Malito E, Shen Y, Rich D, Tang WJ, Kent SB
J Am Chem Soc. 2007 Sep 19;129(37):11480-90

STRUCTURE OF SUBSTRATE-FREE HUMAN INSULIN-DEGRADING ENZYME (IDE) AND BIOPHYSICAL ANALYSIS OF ATP-INDUCED CONFORMATIONAL SWITCH OF IDE 
Im H*, Manolopoulou M*, Malito E*, Shen Y, Zhao J, Neant-Fery M, Sun CY, Meredith SC, Sisodia SS, Leissring MA, Tang WJ
(*equally contributed) 
J Biol Chem. 2007 Aug 31;282(35):25453-63


2006


ELUCIDATION OF HUMAN CHOLINE KINASE CRYSTAL STRUCTURES IN COMPLEX WITH THE PRODUCTS ADP OR PHOSPHOCHOLINE 
           Malito E, Sekulic N, Too WC, Konrad M, Lavie A
J Mol Biol. 2006 Nov 24;364(2):136-151 


2005


BAEYER-VILLIGER MONOOXYGENASES: FROM GENOME ANALYSIS TO STRUCTURAL DETAILS 
Fraaije MW, Torres Pazmino DE, Malito E, Mattevi A, Janssen DB
J Biotechnol. 2005 Aug 1;118(S1):S88-S88


2004


CRYSTAL STRUCTURE OF A BAEYER-VILLIGER MONOOXYGENASE 
           Malito E, Alfieri A, Fraaije MW, Mattevi A
Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13157-62

STRUCTURES OF MICHAELIS AND PRODUCT COMPLEXES OF PLANT CYTOKININ DEHYDROGENASE: IMPLICATIONS FOR FLAVOENZYME CATALYSIS 
           Malito E, Coda A, Bilyeu KD, Fraaije MW, Mattevi A
J Mol Biol. 2004 Aug 27;341(5):1237-49