Crystallography‎ > ‎Crystals‎ > ‎

Random notes


- ISOTROPIC = Cubic (point-group symmetry)
- UNIAXIAL = Trigonal, Tetragonal, Hexagonal
- BIAXIAL = Orthorhombic, Monoclinic, Triclinic

In-situ proteolysis


[...] add commercially produced chymotrypsin to the crystallization trials at a starting ratio of 1:100 chymotrypsin to target protein. If chymotrypsin fails, they try trypsin, elastase, papain, subtilisin A and endoprotease Glu-C (V8 protease), at differing ratios.

From: Crystals from recalcitrant proteins http://kb.psi-structuralgenomics.org/update/2009/08/full/th_psisgkb.2009.36.html

Other links:

pH and Protein Crystallization

From Hampton @ http://hamptonresearch.com/tips_a_to_z.aspx?l=p

Radiation damage

- An interesting document showing flux densities for different beamlines (useful for planning crystal diffraction experiments):
http://bl831.als.lbl.gov/~jamesh/ACA2007/damage_rates.pdf

- An interesting application to calculate how long a particular crystal composition will behave at a particular wavelength:
http://scripts.iucr.org/cgi-bin/paper?ba5146

- A paper showing how radiation damage can be exploited to solve crystal structures:
http://www.cell.com/structure/abstract/S0969-2126(03)00006-6